Researchers from the Technische Universitaet Muenchen and the Juelich Research Center have created the first of its kind graphene transistor array capable of recording signals generated by living cells, with good spatial and temporal resolution.
The proof-of-concept platform opens the way for further investigation of a promising new material. Graphene's distinctive combination of characteristics makes it a leading contender for future biomedical applications requiring a direct interface between microelectronic devices and nerve cells or other living tissue.
The experimental setup reported in Advanced Materials began with an array of 16 graphene solution-gated field-effect transistors (G-SGFETs) fabricated on copper foil by chemical vapour deposition and standard photolithographic and etching processes.
Directly on top of this array, the researchers grew a layer of biological cells similar to heart muscle. Not only were the "action potentials" of individual cells detectable above the intrinsic electrical noise of the transistors, but these cellular signals could be recorded with high spatial and temporal resolution. For example, a series of spikes separated by tens of milliseconds moved across the transistor array in just the way action potentials could be expected to propagate across the cell layer. Also, when the cell layer was exposed to a higher concentration of the stress hormone norepinephrine, a corresponding increase in the frequency of spikes was recorded.
Today, if a person has an intimate and dependent relationship with an electronic device, it's most likely to be a smart phone; however, much closer connections may be in store in the foreseeable future. For example, "bioelectronic" applications have been proposed that would place sensors and in some cases actuators inside a person's brain, eye, or ear to help compensate for neural damage. Pioneering research in this direction was done using the mature technology of silicon microelectronics, but in practice that approach may be a dead end: Both flexible substrates and watery biological environments pose serious problems for silicon devices; in addition, they may be too "noisy" for reliable communication with individual nerve cells.
Of the several material systems being explored as alternatives, graphene – essentially a two-dimensional sheet of carbon atoms linked in a dense honeycomb pattern – seems very well suited to bioelectronic applications: It offers outstanding electronic performance, is chemically stable and biologically inert, can readily be processed on flexible substrates, and should lend itself to large-scale, low-cost fabrication. The latest results from the TUM-Juelich team confirm key performance characteristics and open the way for further advances toward determining the feasibility of graphene-based bioelectronics.
Source:Technische Universitaet Muenchen and the Juelich Research Center
Dr. Jose Antonio Garrido, a member of the Walter Schottky Institute at TUM, said, “The sensing mechanism of these devices is rather simple.”, and added, “Variations of the electrical and chemical environment in the vicinity of the FET gate region will be converted into a variation of the transistor current. Garrido explained that “Much of our ongoing research is focused on further improving the noise performance of graphene devices. And on optimizing the transfer of this technology to flexible substrates such as parylene and kapton, both of which are currently used for in vivo implants. We are also working to improve the spatial resolution of our recording devices.”
The researchers are also working with scientists at the Paris-based Vision Institute to investigate the biocompatibility of graphene layers in cultures of retinal neuron cells, as well as within a broader European project called NEUROCARE, which aims at developing brain implants based on flexible nanocarbon devices.
Research paper: Graphene Transistor Arrays for Recording Action Potentials from Electrogenic Cells; Advanced Materials 2011, 23, 5045-5049. DOI: 10.1002/adma.201102990